SrasV1, Main, Exploration, bibRecord, 005B36

False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide

Identifieur interne : 005B36 ( Main/Exploration ); précédent : 005B35; suivant : 005B37

False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide

Auteurs : Patrick C. Y. Woo [République populaire de Chine] ; Susanna K. P. Lau [République populaire de Chine] ; Beatrice H. L. Wong [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; Wai-Ting Hui [République populaire de Chine] ; Grace S. W. Kwan [République populaire de Chine] ; J. S. Malik Peiris [République populaire de Chine] ; Robert B. Couch [États-Unis] ; Kwok-Yung Yuen [République populaire de Chine]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.

RBID : Pascal:05-0052213

Descripteurs français

KwdFr :
- Anticorps antiviraux (sang), Faux positifs, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Peptides (génétique), Peptides (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Réactions croisées, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Technique de Western, Test ELISA, Virus du SRAS (immunologie).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines nucléocapside.
- immunologie : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines recombinantes, Virus du SRAS.
- sang : Anticorps antiviraux.
- virologie : Syndrome respiratoire aigu sévère.
Pascal (Inist)
- Faux positifs, Glycoprotéine de spicule des coronavirus, Humains, Nucléocapside, Réactions croisées, Technique ELISA, Méthode immunoblotting, Polypeptide, Microbiologie, Syndrome respiratoire aigu sévère, Technique de Western, Test ELISA, Virus syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Antibodies, Viral (blood), Blotting, Western, Cross Reactions, ELISA assay, Enzyme-Linked Immunosorbent Assay, False Positive Reactions, Humans, Immunoblotting assay, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Microbiology, Nucleocapsid, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Peptides (genetics), Peptides (immunology), Polypeptide, Recombinant Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Membrane Glycoproteins, Nucleocapsid Proteins, Peptides, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Nucleocapsid Proteins, Peptides, Recombinant Proteins, Viral Envelope Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Blotting, Western, Cross Reactions, Enzyme-Linked Immunosorbent Assay, False Positive Reactions, Humans, Spike Glycoprotein, Coronavirus.

Abstract

Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.

Affiliations:

Le document en format XML

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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term>
<term>Blotting, Western</term>
<term>Cross Reactions</term>
<term>ELISA assay</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>False Positive Reactions</term>
<term>Humans</term>
<term>Immunoblotting assay</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Microbiology</term>
<term>Nucleocapsid</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Peptides (genetics)</term>
<term>Peptides (immunology)</term>
<term>Polypeptide</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term>
<term>Faux positifs</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Humains</term>
<term>Peptides (génétique)</term>
<term>Peptides (immunologie)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Réactions croisées</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Peptides</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Peptides</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines nucléocapside</term>
<term>Protéines recombinantes</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term>
<term>Cross Reactions</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>False Positive Reactions</term>
<term>Humans</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Faux positifs</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Nucléocapside</term>
<term>Réactions croisées</term>
<term>Technique ELISA</term>
<term>Méthode immunoblotting</term>
<term>Polypeptide</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Virus syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Texas</li>
</region>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name>
</noRegion>
<name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<name sortKey="Hui, Wai Ting" sort="Hui, Wai Ting" uniqKey="Hui W" first="Wai-Ting" last="Hui">Wai-Ting Hui</name>
<name sortKey="Kwan, Grace S W" sort="Kwan, Grace S W" uniqKey="Kwan G" first="Grace S. W." last="Kwan">Grace S. W. Kwan</name>
<name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name>
<name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name>
<name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
</country>
<country name="États-Unis"><region name="Texas"><name sortKey="Couch, Robert B" sort="Couch, Robert B" uniqKey="Couch R" first="Robert B." last="Couch">Robert B. Couch</name>
</region>
</country>
</tree>
</affiliations>
</record>

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False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide

False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide

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